Morphogenesis features of <i>Hedysarum alpinum</i> L. in <i>in vitro</i> culture

The plants of Hedysarum alpinum L. in the North are characterized by a long pregenerative period, laboriousness seed propagation, low field germination seeds, rare self-sowing, and impossibility vegetative propagation. In connection with this, it seemed relevant to introduce this species (included Red Book Komi Republic) into aseptic culture preserve maintain gene pool, as well further develop technology its cultivation obtain medicinal raw materials. morphogenesis was studied, peculiarities regeneration adventitious shoots vitro were revealed. During three subcultivations, influence two cytokinin growth regulators, 6-benzylaminopurine (BAP) kinetin (KIN), at concentrations from 0,5 1,0 mg/l, on potential different explants determined WPM environment. A high frequency single-node segments cotyledon nodes obtained 79% 65%, respectively. second third passages, there an increase regenerative activity node multiplication factor 5 per explant. It shown that relatively (1,0 mg/l) BAP CIN number explant but inhibit their development. For reproduction stage proper, medium containing + IAA 0,1 mg/l is optimal. initiation rhizogenesis actively took place supplemented IBA which proportion rhizogene 77%. maximum values for length roots combined use auxins (IAA mg/l). regenerated transplanted non-sterile conditions, survival rate under ex conditions 50%.